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. 2007 Jul;121(3):377–382. doi: 10.1111/j.1365-2567.2007.02585.x

Figure 4.

Figure 4

(a) PBMC were fixed and permeabilized in paraformaldehyde solution and then incubated with PV or normal IgG. Binding of the antibodies on PBMC surface was revealed by FITC-conjugated anti-human IgG. Only PV IgG stained all around cell membrane of PBMC. The right-hand panels represent magnifications of the highlighted boxes in the left panels. (b) Pre-incubation of PV serum with purified keratinocyte 130 000 protein(s) abolished the staining of PV IgG on PBMC surface (left panel), whereas preincubation with gel-pure 130 000 protein(s) from PBMC extracts apparently did not impaire the ability of PV IgG to recognize keratinocyte antigens. The figure is representative of at least two independent experiments.