Table 1.
Expression of CXCR6 on normal rat blood, lymph node (LN) and spleen cells, and on in vivo antigen-challenged LN lymphoblasts
| Blood lymphocytes | LN lymphocytes | Spleen lymphocytes | LN lymphoblasts | |||||
|---|---|---|---|---|---|---|---|---|
| % positive | MFI | % positive | MFI | % positive | MFI | % positive | MFI | |
| CD4+ | 3·8 ± 0·7 | 11·2 ± 2·6 | 2·8 ± 0·7 | 14·3 ± 3·0 | 3·8 ± 0·6 | 12·0 ± 0·9 | 6·7 ± 1·3* | 9·1 ± 0·6 |
| CD8+ | 4·4 ± 1·0 | 7·2 ± 0·7 | 3·1 ± 1·0 | 9·9 ± 1·4 | 6·3 ± 1·4 | 10·2 ± 0·7 | 10·0 ± 3·1* | 8·2 ± 0·5 |
| CD45RC+ | 0·9 ± 0·3 | 8·9 ± 2·1 | 0·9 ± 0·2 | 11·8 ± 1·4 | 1·6 ± 0·5 | 10·9 ± 0·5 | 1·2 ± 0·6 | 15·3 ± 2·8 |
| CD45RC– | 8·2 ± 1·8 | 9·7 ± 0·8 | 5·1 ± 1·2 | 17·3 ± 1·4 | 8·0 ± 1·9 | 12·8 ± 0·9 | 9·5 ± 0·7** | 12·0 ± 0·9 |
| CD25+ | 12·3 ± 2·6 | 9·8 ± 0·7 | 12·3 ± 2·0 | 20·2 ± 3·4 | 15·2 ± 1·8 | 12·7 ± 1·1 | 21·3 ± 4·3* | 10·4 ± 0·6 |
| NK T cells | 7·3 ± 1·9 | 8·8 ± 1·1 | 10·2 ± 3·2 | 12·7 ± 3·5 | 9·8 ± 3·1 | 11·1 ± 0·7 | 14·1 ± 3·2 | 9·9 ± 0·7 |
| NK cells | 17·1 ± 3·5 | 15·3 ± 3·1 | 22·3 ± 4·3 | 14·8 ± 4·2 | 20·3 ± 5·3 | 11·5 ± 0·6 | 31·6 ± 5·0 | 10·1 ± 0·7 |
| CD62L+ | 2·2 ± 0·8 | 7·7 ± 1·0 | 1·9 ± 0·6 | 13·8 ± 1·7 | 1·7 ± 0·3 | 11·4 ± 0·7 | 8·5 ± 2·6* | 9·2 ± 0·7 |
| CD62L– | 6·5 ± 1·8 | 9·2 ± 0·9 | 4·0 ± 1·3 | 16·7 ± 2·9 | 4·9 ± 1·1 | 11·8 ± 0·9 | 11·3 ± 3·4* | 10·0 ± 0·5 |
Rat blood, LN and spleen cells were isolated and stained as outlined in the Materials and methods section. LN lymphoblasts were obtained from draining LNs of animals immunized 4 days previously in the footpads with vaccinia virus. Each entry shows the mean ± standard error of the mean (n = 6–8).
*
P < 0·05
**
P < 0·01, compared to normal LN T cells.
MFI, mean fluorescence intensity.