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. 2007 Sep;122(1):80–89. doi: 10.1111/j.1365-2567.2007.02615.x

Figure 6.

Figure 6

The up-regulation of TLR2 is delayed following colonization with the carrier strain of S. aureus. (a) NEC layers were cultured at the air–liquid interphase in an antibiotic-free medium and inoculated with 20 ± 1 (mean ± SEM) bacteria/well for a period of up to 20 hr. The organisms used were S. aureus D30 (SA D30), the carrier strain; S. aureus 930918 (SA 930918), the non-carrier strain; and S. epidermidis (SE), the control strain. Expression of TLR2 mRNA from NEC was assessed by real-time quantitative RT-PCR (qPCR). GAPDH was used to normalize cDNA input. The results represent mean ± SEM of four separate experiments. (b,c) Representative acrylamide gel (0·5 μg/ml ethidium bromide) of TLR2 (b) and GAPDH (c) qPCR products visualized under UV after 40 rounds of amplification to verify that the qPCR reactions amplified only the products of interest.