Figure 6.
(a) Representative reactivity pattern of an anti-huCol V hyperimmune rabbit in HEp-2 cells, by immunoblot. Lane a: serum from a rabbit injected with Freund's complete adjuvant (FCA), at day 75; lanes b, c, d, e: sera from a hyperimmune rabbit obtained on days 0, 30, 75, and 120 after immunization; lane f: anti-Scl70 human serum; lane g: serum from a BSA-immunized rabbit (75 days). (b) Lanes a and b: Anti-RNA polymerase I human sera; lane c: anti-huCol V rabbit serum (75 days after immunization).