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Reactivity pattern of an anti-huCol V hyperimmune rabbit serum against cytoplasmic-enriched fraction of HEp-2 cells by immunoblot. Lanes a and f: normal rabbits; lanes b and d: huCol V rabbits' sera, obtained on day 75 after primary immunization; lanes c and e: two representative anti-Golgi human sera; lane g: control serum from rabbit injected with FCA (75 days).
