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The effects of SMA-7 and lactacystin (LC) on the LPS-induced activation of NF-κB in macrophages. Cells were incubated for 3 hr with LPS alone or with LPS plus SMA-7 or LC. Nuclear extracts were prepared and subjected to the electrophoretic mobility shift assay of NF-κB described in the Materials and methods. Similar results were obtained in two independent experiments.
