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The effect of SMA-7 on the acid (a) and neutral (b) SMase activities in macrophages treated with TNF-α. Cells were incubated at 37° for set periods ranging from 0 hr to 24 hr in serum-rich medium with TNF-α (20 ng/ml) alone or with TNF-α plus 10 μm SMA-7. Each bar represents the mean ± SD of three independent experiments. **P < 0·01; ***P < 0·005, compared to the control. ##P < 0·01; ###P < 0·005, compared to TNF-α alone.
