Table 1.
Proteasomal cleavage of insulin oxidized B chain1
| Cleavage after | Non-infected yield (%) | Infected yield (%) | Ratio non-infected : infected |
|---|---|---|---|
| Phe1 | Not observed | Not observed | – |
| Val2 | Not observed | Not observed | – |
| Asn3 | Not observed | Not observed | – |
| Gln4 | 31·3 | 24·2 | 1·3 |
| His5 | 7·0 | 10·0 | 0·7 |
| Leu6 | 11·2 | 11·0 | 1·0 |
| Cys7 | 0·1 | 0·2 | 0·5 |
| Gly8 | Not observed | Not observed | – |
| Ser9 | 0·2 | 0·3 | 0·7 |
| His10 | 0·1 | 0·1 | 1·0 |
| Leu11 | 0·8 | 0·9 | 0·9 |
| Val12 | Not observed | Not observed | – |
| Glu13 | 16·8 | 15·1 | 1·1 |
| Ala14 | 1·5 | 1·6 | 0·9 |
| Leu15 | 5·7 | 4·2 | 1·4 |
| Tyr16 | Not observed | Not observed | – |
| Leu17 | 10·7 | 9·9 | 1·1 |
| Val18 | 3·6 | 4·2 | 0·9 |
| Cys19 | 8·4 | 9·0 | 0·9 |
| Gly20 | 0·4 | 0·6 | 0·7 |
| Glu21 | 0·1 | 0·2 | 0·5 |
| Arg22 | Not observed | Not observed | – |
| Gly23 | 0·1 | Not observed | – |
| Phe24 | 5·4 | 6·2 | 0·9 |
| Phe25 | Not observed | 0·1 | – |
| Tyr26 | 2·8 | 2·4 | 1·2 |
| Thr27 | 0·1 | Not observed | – |
| Pro28 | 0·2 | 0·1 | 2 |
| Lys29 | 1·1 | 1·0 | 1·1 |
1
Cleavage yield at a peptide bond was estimated as the total percentage of peptides in the digestion mixture resulting from cleavage at that bond.