Figure 3.
Analysis of peripheral blood T lymphocyte populations by flow cytometry. The acquisition panels represent T cells gated on the CD19-negative, CD45-positive population. Cells were incubated with anti-5-LOX monoclonal antibody and F(ab)′2 goat anti-mouse fluorescein isothiocyanate (FITC) followed by staining with anti-CD3 (a), CD4 (b), CD8 (c), CD2 (d), CRTH2 (e), CD45RA (f), CD45RO (g), TCR-αβ (h), TCR-γδ (i), CD1a (j), CD7 (k) and CD10 (l) antibodies. The x-axis represents 5-LOX FITC and the y-axis represents the antibodies defining the different subpopulations. Results are from one experiment and are representative of five.