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. 2007 Nov;122(3):409–417. doi: 10.1111/j.1365-2567.2007.02655.x

Figure 5.

Figure 5

T1IFN-β induced semi-maturation of resting DCs. (a) T1IFN-β up-regulated the expression of CD1d and maturation markers (CD80, CD40) on resting immature DCs. Myeloid DCs were derived from PBMC of healthy individuals with rhGM-CSF (400 U/ml) and rhIL-4 (200 U/ml) in the presence (open histogram) or absence (shaded histogram) of rhT1IFN-β (1000 U/ml). Expression of CD11c, maturation markers CD80 and CD40 and CD1d was determined by flow cytometric analysis. CD80, CD40 and CD1d histograms refer to gated CD11c+ cells. The graph at the right side of each histogram represents cumulative data of three separate determinations ±SEM. (b) T1IFN-β-conditioned DCs were not fully mature and did not secrete cytokines (IL-12 and IL-10). Cytokine release by immature DCs derived from PBMC without (iDC) or with rhT1IFN-β (T1IFN-β iDC) was assessed by CBA on supernatants of 5-day DC cultures. As positive controls, we used fully mature DCs (mDC) obtained by adding LPS (1 μg/ml) to the last 24 hr of culture.