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. 2007 Nov;122(3):394–400. doi: 10.1111/j.1365-2567.2007.02653.x

Figure 4.

Figure 4

Autoantibody production is significantly enhanced in MRL-Faslpr (lpr)/4-1BB–/– mice. (a–c) Three and five months after initial treatment, mice were killed, and sera were collected and applied at indicated dilutions to HEp-2-coated slides for the analysis of serum antinuclear antibody titres; bound immune complexes were visualized using appropriate fluorescein isothiocyanate (FITC)-conjugated anti-immunoglobulin antibodies as mentioned in the Materials and methods. (d) Sera (diluted 1/500) from 5-month-old mice were applied to appropriate anti-immunoglobulin coated microtitre wells and levels of circulating autoantibodies were evaluated by enzyme-linked immunosorbent assay (ELISA) using commercial kits. (e) Sera (diluted 1/500) from 5-month-old mice were incubated in microtitre plates precoated with double-stranded DNA (dsDNA). Immune complexes formed were quantified by ELISA. Magnification ×9·6. OD, optical density.