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Antigen-induced IFN-γ and TNF production by splenocytes from 4 weeks BCG-infected mice. IFN-γ (a and b) and TNF (c and d) were evaluated in the culture supernatant from cells incubated with medium (non treated: NT), or activated with BCG culture filtrate proteins (BCG antigens: Ag) (a and c) or with viable BCG (BCG) (b and d). Values are represented as means ± SEM from four to five mice/group (assay in duplicate). *P < 0·05.
