Table 1.

Administration techniques for targeting the different sites of the respiratory tract

Targeted site of deposition	Delivery route	Vol. (μl) solution delivered	Angle of tilt of mouse	Air bolus
Nasal passages	i.n.	10	90°	none
Upper airways	i.n.	50	90°	none
Central airways	i.t.	10	0°	none
Deep lung	i.t.	25	45°	1

Targeting was achieved by varying the delivery route, the volume of solution and the position of the mouse and by the possible insufflation of a 200 μl air bolus following administration.