Figure 5.

Activation of p38 MAPK, ERK1,2 and JNK1,2 in BEAS-2B cells. BEAS-2B cells (1 × 10⁶/well) were cultured with or without IL-31 (100 ng/ml) for different incubation times as indicated. Total cellular proteins were extracted from the cells for the measurement of (a) phosphorylated and total p38 MAPK, (b) phosphorylated and total ERK1,2 and (c) phosphorylated and total JNK1,2 proteins by Western blot analysis. Experiments were performed in three independent replicates with essentially identical results, and representative results are shown. Total p38 MAPK, ERK1,2, and JNK1,2 were used as protein control to ensure equal protein loading.