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. 2007 Dec;122(4):476–485. doi: 10.1111/j.1365-2567.2007.02674.x

Figure 5.

Figure 5

CIITA siRNA inhibited expression of CIITA and HLA-DR in both Th1 and Th2 cells. (a) In contrast to the negative effect of CIITA mRNA in naive Th (NA) cells, a higher transcription level of CIITA mRNA was detected in the in vivo differentiated Th1 cells than in Th2 cells as determined by regular RT-PCR. (b) Unstimulated naive T cells were transfected with control siRNA (CON si) or CIITA siRNA (CIITA si) and then stimulated with anti-CD3 (1 μg/ml) plus anti-CD28 (2 μg/ml) for 72 hr in Th1 or Th2 differential conditions, and the effects of siRNA on CIITA expression in differentiated Th1 or Th2 cells were evaluated by Western blotting. It was shown that siRNA efficiently inhibited CIITA expression in both Th1 and Th2 cells. (c) Flow cytometric analysis indicated that NA cells did not express surface HLA-DR. Th1 and Th2 cells could express comparable levels of HLA-DR molecules, which was significantly inhibited by introduction of CIITA siRNA.