FIGURE 11. OA-NO2 induces [3H]2-deoxy-D-glucose uptake in differentiated 3T3-L1 adipocytes.
A, 3T3-L1 preadipocytes were differentiated to adipocytes and treated with OA-NO2 or LNO2 for 2 days prior to addition of [3H]2-deoxy-D-glucose. OA-NO2 (1 μM) induced significant increases in glucose uptake, with this effect paralleled by LNO2 (3 μM; p ≤ 0.05; Student’s t test). B, the increased adipocyte glucose uptake induced by nitroalkenes and the positive control rosiglitazone were significantly inhibited by the PPARγ-specific antagonist GW9662 (p ≤ 0.05; Student’s t test). All values are expressed as mean ± S.D. (n = 3).