. Author manuscript; available in PMC: 2008 Mar 10.

Published in final edited form as: J Biol Chem. 2005 Oct 14;280(51):42464–42475. doi: 10.1074/jbc.M504212200

TABLE THREE. Nitrated oleic acid in human blood: a comparison with nitrated linoleic acid.

Plasma and red cells obtained from the venous blood from apparently healthy human volunteers and were extracted and prepared for mass spectrometric analysis as described under “Materials and Methods.” During sample preparation, [¹³C]OA-NO₂ was added as an internal standard to correct for losses. OA-NO₂ was quantitated by fitting analyte to internal standard area ratios obtained by MS to an internal standard curve. Concentration values for LNO₂ in the vascular compartment were obtained from ref. 9. Data are expressed as mean ± S.D. (n = 10; 5 female and 5 male).

Compartment	Fraction	[OA-NO₂]	[LNO₂]
Plasma (nM)	Free	619 ± 52	79 ± 35
	Esterified	302 ± 369	550 ± 275
	Total	921 ± 421	630 ± 240
Packed red cells (nM)	Free	59 ± 11	50 ± 17
	Esterified	155 ± 65	199 ± 121
	Total	214 ± 76	249 ± 104
Whole Blood (nM)^a	Total	639 ± 366^a	477 ± 128^a
Urine (fmol/mg creatinine)		5.40 ± 52	2.28 ± 0.84

Assuming a 40% hematocrit.