Figure 2.

Composite tree of ValRS and IleRS using the ML method of protein phylogeny (JTT-F model). A ProtML tree finally selected by the local rearrangement method of the protml program was shown. The horizontal length of each branch is proportional to the estimated number of substitutions. Local bootstrap probabilities calculated by the protml program are attached to the internal branches. Asterisks (★) denote amitochondriate eukaryotes. cyt, Cytosol; mit, mitochondrion; plasmid, gene carried on a plasmid. Unambiguously aligned 270 positions were selected and used. These correspond to the following positions in the ValRS sequence of G. lamblia: 142–143, 145–153, 171–253, 409–438, 469–487, 491–493, 497–525, 554–581, 624–642, 657–666, 669–683, and 686–708.