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(A) Western blot analysis of hMLH1 in colorectal cell lines. Note detectable protein in SW480 and HT29 before drug treatment (AzaC), but in RKO and SW48 only after drug treatment. (B) Demethylation analysis of cell lines after azacytidine treatment. The presence of U product in RKO and SW48 after 5-aza-2′-deoxycytidine indicates the presence of demethylation of the hMLH1 promoter in these cell lines.
