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. Author manuscript; available in PMC: 2008 Mar 10.
Published in final edited form as: FEMS Yeast Res. 2006 Mar;6(2):218–229. doi: 10.1111/j.1567-1364.2006.00031.x

Fig. 5.

Fig. 5

Panels A–F. Effects of rapamycin (200 ng/ml, panels A, C and E) and MSX (2 mM, panels B, D and F) treatment on intracellular Gat1-Myc13 and Gln3-Myc13 distribution (panels A–D) and Gat1-Myc13 phosphorylation (panels E and F) in RJ715 transformed with pGAT1-Myc grown in YNB-ammonia medium. Culture conditions and methods of cell preparation and counting were as described in earlier figures. The times of treatment are indicated below the histograms and above the Western blots, respectively. Panels G and H. Carbon starvation induced Gat1-Myc13 phosphorylation is Snf1-dependent. However, MSX- and carbon starvation induced Snf1-independent phosphorylation observed with Gln3-Myc13 (Tate et al., 2005) was not demonstrable for Gat1-Myc13. Further experimental conditions and Western blot analysis are the same as in earlier figures. pGAT1-Myc transformed RJ715 grown in ammonia-YNB medium were used for preparation of cell extracts. Cells were treated with MSX for 30 min. Strains used in panel H were TB106-2a and RR183.