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. 2005 Oct;126(4):353–363. doi: 10.1085/jgp.200509312

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Copyright © 2005, The Rockefeller University Press

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Figure 4. — (A) HEK 293 cells coexpressed SK3 and EGFP genes used in the study. Green fluorescent cells were chosen to measure SK3 currents (left), and the figure on the right shows the same cells in bright field. (B) UCL 1684 blocks SK3 channels expressed in HEK 293 cells. i and iii traces show the representative currents recorded at −80 mV (i) or +10 mV (iii) from 15 cells. Green fluorescent cells were selected and the SK3 currents were recorded using the whole cell patch clamp configuration. As shown in figure, bath application of an SK blocking solution containing 10 mM BaCl₂ and 30 mM TEA-Cl, resulted in small basal currents. After replacing the external solution with 30 mM KCl, robust SK3 currents are observed (*, P < 0.05; **, P < 0.01, compared with the basal level). 10 nM UCL 1684 significantly inhibited this current at −80 mV (ii) or +10 mV (iiii) (#, P < 0.05; ##, P < 0.01, n = 6, bottom bar graph). (C) SK3-mediated currents in HEK 293 cells are also significantly blocked by AZ (##, P < 0.01, n = 7).

Figure 4. — (A) HEK 293 cells coexpressed SK3 and EGFP genes used in the study. Green fluorescent cells were chosen to measure SK3 currents (left), and the figure on the right shows the same cells in bright field. (B) UCL 1684 blocks SK3 channels expressed in HEK 293 cells. i and iii traces show the representative currents recorded at −80 mV (i) or +10 mV (iii) from 15 cells. Green fluorescent cells were selected and the SK3 currents were recorded using the whole cell patch clamp configuration. As shown in figure, bath application of an SK blocking solution containing 10 mM BaCl₂ and 30 mM TEA-Cl, resulted in small basal currents. After replacing the external solution with 30 mM KCl, robust SK3 currents are observed (*, P < 0.05; **, P < 0.01, compared with the basal level). 10 nM UCL 1684 significantly inhibited this current at −80 mV (ii) or +10 mV (iiii) (#, P < 0.05; ##, P < 0.01, n = 6, bottom bar graph). (C) SK3-mediated currents in HEK 293 cells are also significantly blocked by AZ (##, P < 0.01, n = 7).