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. 2005 Oct;126(4):325–337. doi: 10.1085/jgp.200509334

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Copyright © 2005, The Rockefeller University Press

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Figure 4. — Two-hybrid mapping analyses. Cotransformed yeast were tested for their ability to grow on −UHTL plates (columns +Leu) or generate strong β-gal activity on −UHT + X plates (columns +X-gal). (A) Each cell expresses a different Ct2_NKCC2 prey (among those identified during the initial two-hybrid screen) but the same Ct1_{NKCC2(657–836)} bait. (B) Each cell was cotransformed with a huNKCC1- and a huNKCC2-encoding construct using the Ct1 domain of one isoform and the Ct2 domain of the other isoform. (C) Cells coexpress the protein segments shown in Fig. 3 D along with Ct2_{NKCC2(830–1099)} or Ct1_{NKCC2(657–836)}, respectively. All of the cotransformants were incubated at 30°C for 3–6 d.