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. 2005 Oct;126(4):325–337. doi: 10.1085/jgp.200509334

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Copyright © 2005, The Rockefeller University Press

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Figure 5. — Flux studies. X. laevis oocytes were injected with saNKCC2A, saNKCC2F, saNKCCC2AF, saNKCC2A + saNKCC2AF, or saNKCC2F + saNKCC2AF (∼2 ng for A or F, and ∼20 ng for AF). Groups of eggs were also injected with H₂O alone to determine background cotransporter activity. (A and B) ⁸⁶Rb influx measured in 13–22 oocytes expressed as means ± SEM. Bumetanide and ouabain were added to the influx and wash solution at a concentration of 250 μM and 10 μM. (C) ²²Na influx measured in 9–10 oocytes expressed as means ± SEM. Hydrochlorothiazide, amiloride, and ouabain were added to the influx and wash solution at concentrations of 250, 125, and 125 μM. Here, * indicates that within any given panel, the values are significantly different from those of A or F (P < 0.01).