TABLE I.
Characteristics of pBoc and Intestinal Ca2+ Oscillations and Ca2+ Waves in Control Worms (glo-1) and itr-1 Mutants
| Allele | pBoc period | pBoc CV | Ca2+ oscillation period | Ca2+ oscillation CV | Ca2+ oscillation rise time | Ca2+ oscillation fall time |
|---|---|---|---|---|---|---|
| s | % | s | % | s | s | |
| glo-1 | 55 ± 1 (6) | 4 ± 1 (6) | 49 ± 3 (6) | 16 ± 1 (6) | 8 ± 1 (5) | 23 ± 3 (5) |
| itr-1(sa73) | 111 ± 10a (6) | 9 ± 3 (6) | 271 ± 30a , b (6) | 24 ± 7 (6) | 17 ± 2a (6) | 30 ± 6 (6) |
| itr-1(sy290) | 49 ± 1 (6) | 4 ± 1 (6) | 50 ± 3 (6) | 10 ± 3 (6) | 6 ± 1 (6) | 17 ± 3 (6) |
| itr-1(sy327) | 53 ± 2 (9) | 8 ± 2 (9) | 42 ± 2 (7) | 11 ± 1 (7) | 5 ± 1 (7) | 20 ± 1 (7) |
Values are means ± SEM (n = number of worms or intestines). Fluorescence images were acquired at 1–10-s intervals.
a
P < 0.001 compared with glo-1 control animals.
b
P < 0.002 compared with pBoc in itr-1(sa73) worms.