TABLE IV.
Effect of PMA and Loss of Diacylglycerol Kinase Activity on egl-8(n488)- or egl-8(RNAi)-induced pBoc Arrhythmia
| Experiment | pBoc coefficient of variance (%) |
|---|---|
| egl-8(n488) exposed to 0.1 μM PMAa | 48 ± 8 (6) |
| egl-8(RNAi) | 39 ± 5 (6) |
| dgk-1(sy428) | 12 ± 2 (5) |
| egl-8(RNAi);dgk-1(sy428) | 57 ± 7 (5) |
| dgk-2(gk124) | 7 ± 2 (6) |
| egl-8(RNAi);dgk-2(gk124) | 30 ± 4 (6) |
| dgk-3(gk110) | 14 ± 4b (6) |
| egl-8(RNAi);dgk-3(gk110) | 29 ± 7 (6) |
a
The frequency of pBoc in egl-8(n488) worms exposed to PMA was severely reduced. pBoc rhythm was therefore quantified over a fixed 20-min observation period. In the six worms examined, two to nine pBocs were detected. For all other worms, pBoc rhythm was quantified over 10 cycles. Values are means ± SEM (n = number of worms).
b
P < 0.05 compared with N2/glo-1 control animals.