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. Author manuscript; available in PMC: 2008 Nov 10.

Published in final edited form as: Virology. 2007 Jul 25;368(1):91–101. doi: 10.1016/j.virol.2007.06.023

Fig. 4 — (A). Nuclear extracts were prepared from A549 cells control and infected with hMPV, MOI of 3, for 6, 12, 24 and 48 h and used for binding to the IL-8 NF-kB probe in EMSA. Shown are the nucleoprotein complexes formed on the probe in control cells and in response to the infection. C1 and C2 are hMPV-inducible complexes, while C3 is constitutive. UV indicates UV-inactivated virus. (B) Western blot of p50 and p65 in hMPV-infected cells. Nuclear proteins were prepared from control and A549 cells infected for various length of time, fractionated on a 10% SDS-PAGE, transferred to PVDF membranes and probed with the appropriate antibody. Lamin b was used as an internal control to determine equal loading of the samples.

Fig. 4 — (A). Nuclear extracts were prepared from A549 cells control and infected with hMPV, MOI of 3, for 6, 12, 24 and 48 h and used for binding to the IL-8 NF-kB probe in EMSA. Shown are the nucleoprotein complexes formed on the probe in control cells and in response to the infection. C1 and C2 are hMPV-inducible complexes, while C3 is constitutive. UV indicates UV-inactivated virus. (B) Western blot of p50 and p65 in hMPV-infected cells. Nuclear proteins were prepared from control and A549 cells infected for various length of time, fractionated on a 10% SDS-PAGE, transferred to PVDF membranes and probed with the appropriate antibody. Lamin b was used as an internal control to determine equal loading of the samples.