Figure 2.

Halo assays of wild-type and congenic yeast strains. Shown are the results of halo assays performed on a wild-type strain (yVT8) and congenic yeast strains harboring disruptions of either the YBR059C gene or YGR179C gene (strains yVT76 and yVT80, respectively) after 4 days. The sensitivity to α factor of the mutant strains relative to the wild-type strain was determined by measuring halo diameters caused by different α factor concentrations and then comparing diameter widths between the strains. The amounts of α factor spotted to the disks at corresponding positions on each plate are shown on the schematic to the left.