Table 1.

Perturbagens

Perturbagens	Chromosome	Position	GFP fusion partner
pep1(I)	NA	NA	15-aa peptide (internal)
pep2(I)	NA	NA	13-aa peptide (internal)
pep4(C)	12	345,284–345,535	59-aa peptide
pep4(C)	13	17,605–17,785	21-aa peptide
pep5(C)	7	954,846–955,084	31-aa peptide
orf1	12	849,840–850,463	amino acids 14–221 of STE11
orf2	3	63,438–64,244	amino acids 32–279 of STE50
orf3	2	329,957–329,565	amino acids 32–160 of GIP1
orf4	5	425,915–424,661	amino acids 1512–1753 of PMD1
orf5	5	408,993–408,253	amino acids 23–269 of YER124C
orf6	2	600,538–600,774	amino acids 11–89 of YBR186W
orf7	13	441,164–443,186	amino acids 653–960 of YMR086W
orf8	2	357,343–355,292	amino acids 934–1108 of YBR059C
orf9	7	854,410–854,195	amino acids 161–231 of YGR179C
orf10	14/5	409,769–409,846/	amino acids 477–502 of YNL115+
		81,490–81,418	amino acids 37–395 of RAD23
orf11	2	390,347–390,624	18-aa peptide + amino acids 26–98 of ECM8

Shown are the chromosomes from which each perturbagen-encoding sequence was derived (Chromosome), the numerical positions of the first and last nucleotides of each genomic sequence fused to GFP (Position, see ref. 8), and the predicted GFP-fused peptides comprising each perturbagen (GFP fusion partner). Sequences of the two internal peptides are as follows: pep1(I), WKYIRCIMPWHRFWF and pep2(I), RGYFNDRWYGCPG. The orf10 perturbagen is composed of tandemly ligated genomic fragments from YNL116W and RAD23 (56), both of which are required for the perturbagen to function (data not shown). The orf11 insert contains a deletion of the G residue present in the genomic sequence at position 390,403. This deletion restores the reading frame of the ECM8 gene (36).