Figure 7.

Confocal reflectance images (left) and bright field images (right) of (a) SKBR3 cells incubated with silver-stained HER81/gold sphere conjugates, (b) CHO cells under the same conditions. Care was taken to maintain the same acquisition parameters in both cases. The silver-stained bioconjugates are detected at the cell membranes of SKBR3 cells where HER2 is localized. This indicates successful conjugation and retention of functionality of the antibody after conjugation. No such accumulation of HER81/gold sphere conjugates is demonstrated in HER2 negative CHO cells.