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. Author manuscript; available in PMC: 2009 Feb 15.
Published in final edited form as: Dev Biol. 2007 Dec 15;314(2):362–375. doi: 10.1016/j.ydbio.2007.12.007

Fig. 6. Analysis and characterization of EGFP positive cells isolated from metrial glands of wild-type females mated to chβA-EGFP transgenic males.

Fig. 6

Panel A, Fluorescence profile of all cells isolated from the metrial gland. Panel B, Fluorescence profile of an aliquot of GFP negative cells; Panel C, Fluorescence profile of an aliquot of GFP positive cells; Panel D, cytokeratin immunostaining of GFP positive cells. Panel E, RT-PCR analysis of Prl7b1, Ascl2, Gcm1, and 18S ribosomal RNA in EGFP positive cells. Abbreviations; MG, metrial gland; LZ, labryrinth zone; JZ, junctional zone; IT, isolated invasive trophoblast. Panel F, Ploidy analysis of EGFP positive invasive trophoblast cells. Panel f-1, total metrial gland cells sorted by fluorescence; panel f-2, spleen cells stained with propidium iodide and analyzed for DNA content; panel f-3, EGFP positive invasive trophoblast cells stained with propidium iodide and analyzed for DNA content.