Table 2.

AFLP primer and adapter sequences

Adapters	5′-Sequence-3′
EcoRI forward adapter	CTC GTA TAC TGC GTA CC
EcoRI reverse adapter	AAT TGG TAC GCA GTA
Msel forward adapter	GAC GAT GAG TCC TGA G
Msel reverse adapter	TAC TCA GGA CTC ATC
+ 1 Pre-selective primers
EcoRI + A	TAC TGC GTA CCA ATT C – A
Msel + C	GAC GAT GAG TCC TGA GTA A – C
+ 3 Selective primers
Msel + CAA (I)	GAC GAT GAG TCC TGA GTA A – CAA
Msel + CAC (III, IV)	GAC GAT GAG TCC TGA GTA A – CAC
EcoRI + AGC (A)	(FAM) - TAC TGC GTA CCA ATT C – AGC
EcoRI + ACG (B)	(HEX) - TAC TGC GTA CCA ATT C – ACG
EcoRI + AAC (C)	(HEX) - TAC TGC GTA CCA ATT C – AAC

Four + 3 selective amplifications, designated IA, IIIA, IIIB, and IVC, were performed. Roman numerals represent non-labeled selective primers and the letters “A, B, or C” represent the 5′ FAM or 5′ HEX labeled primers with bold-type indicating selective nucleotides.