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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Neurobiol Dis. 2007 Sep 19;29(2):278–292. doi: 10.1016/j.nbd.2007.09.002

Fig. 2. Arachnoidal and meningioma cell morphology.

Fig. 2

(A) Phase-contrast (top panels) and confocal (bottom panels) images of patient-matched arachnoidal, AC006, and merlin(-) meningioma, MN302(-), cells at early passages, p3 and p2, respectively. Phase-contrast images were taken from live cells plated in tissue culture plates, and confocal images were taken from fixed cells plated on glass coverslips and stained immunocytochemically with Alexa Fluor-conjugated phalloidin. Arachnoidal cells possessed a more epithelial-like morphology whereas merlin(-) meningioma cells exhibited long cell processes and increased granules. Bar, 100 μm. (B) Phase-contrast images of patient-matched arachnoidal, AC006 p7, and merlin(-) meningioma cells, MN302(-) p6, and unmatched merlin(+) meningioma cells, MN354(+) p6. Merlin(-) meningioma cultures are enlarged and flattened, and characterized by increased granularity and vacuoles that are not prominent features of arachnoidal and merlin(+) meningioma cells at similar passages. Bar, 50 μm.