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. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Neurobiol Dis. 2007 Sep 19;29(2):278–292. doi: 10.1016/j.nbd.2007.09.002

Fig. 3. Cell growth characteristics of merlin(-) meningioma and arachnoidal cells in culture.

Fig. 3

Phase-contrast images of matched pair AC006 and MN302 at various cell passages. (A) Arachnoidal AC006 p15 cells formed confluent cell monolayers, while matched merlin(-) meningioma MN302(-) p4 cells continued to grow after saturating. Bar, 100 μm. Inset, 2X (B) MN302(-) p9 cell cultures, predominantly populated by enlarged, flattened cells, stained positively for the senescence-associated biomarker β-galactosidase (SA-β-Gal). In contrast, AC006 p10 cells were negative, or weakly positive for expression of SA-β-Gal. Bar, 100 μm. (C) MN302(-) cells at p9 were unable to reach cell confluency, unlike matched arachnoidal cells that proliferated steadily and formed confluent monolayers after extensive cell passaging (p26). Merlin(-) meningioma cultures (MN302 p9) developed refractive foci of rapidly growing cells demonstrating loss of cell contact inhibition of growth among slow-growing senescent-like cells. Bar, 100 μm.