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. 2008 Feb 19;98(5):941–949. doi: 10.1038/sj.bjc.6604268

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Copyright © 2008 Cancer Research UK

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RT–PCR analysis (A) and western blot analysis (B) for Bcl-2 and IAP family members in the Caki1 parental cells, mock-transfected cells, and clone nos. 14 and 19. β-Actin was used as a control for loading. The messengers of all examined genes were present in the cells and remained unchanged. Apoptosis-related proteins were also not changed except cIAP-1 and cIAP-2, which were slightly increased in mock transfectants and clone nos. 14 and 19, which presumably reflects the effect of cellular stress during transfection and selection process.