Figure 5.

Depletion of hY3 RNA is cytostatic and does not cause cell death. (A) Cell viability assay. RNAi was performed on WI38 lung fibroblasts and HeLa cervical carcinoma cells. At 0 h and 48 h post transfection with the indicated siRNAs, percentages of viable cells were determined by measuring exclusion of the dye, trypan blue. (B) Cell morphology. Representative phase contrast micrographs of cells are shown at 48 h after transfection. The mitotic index (%M) for each of these cell populations was measured by counting >800 cells per sample and it is indicated at the bottom left of each field. Scale bar, 10 μm.