Table 2.

Whole Blood Rapamycin Levels

	24 hours			48 hours
Treatment Groups	Rapamycin Level (ng/ml)	p value*	Number of Mice	Rapamycin Level (ng/ml)	p value**	Number of Mice
Treatment Trial (Tumor bearing)
0.4% Direct Topical Rapamycin	6.3 ± 0.6	0.02	3	2.3 ± 0.4	0.04	3
0.8% Indirect Topical Rapamycin	11.9 ± 3.2	0.92	3	5.3 ± 0.8	0.25	3
0.8% Direct Topical Rapamycin	12.3 ± 1.5	N/A	3	4.1 ± 0.5	N/A	3
0.16 mg Rapamycin IP	55.9 ± 2.7	0.0005	2	17.4 ± 2.2	0.005	2
Non-Tumor bearing
0.4% Topical Rapamycin (1 dose)	5.9 ± 0.5	0.01	3	1.7 ± 0.9	0.07	3
0.8% Topical Rapamycin (1 dose)	13.0 ± 2.5	0.81	3	4.1 ± 2.3	0.98	3
0.8% Topical Rapamycin + Bandage (1 dose)	3.4 ± 0.6	<0.0001	8	2.6 ± 0.7	0.28	8
0.8% Topical Rapamycin + Collar (3 doses)	21.7 ± 3.7	0.13	6	Not done	N/A	Not done

* Compared to 0.8% direct treatment (tumor bearing) 24 hr blood level.

** Compared to 0.8% direct treatment (tumor bearing) 48 hr blood level