Table 2.
PCR-allelotyped primers predicted by pompous for region 3p21.3
| Oligo name* | Forward primer† | Reverse primer† | Size | Sequence |
|---|---|---|---|---|
| LUCA1.1 | CCTCATCCTCTCTGTTGGG | GCAGAATGACGTGAACCC | 266 | (ct)15 |
| LUCA1.2 | TTCCTCCACAGATTCCTCTG | TTGAGAAGGATGTGGATGAC | 277 | (ca)18 |
| LUCA1.3 | GTTCATGATGGCAGACTCTG | CCCAGAACAATCACAAGATG | 259 | (ca)23 |
| LUCA2.1 | GCTCCTCAGGCAGAGTCC | CCACAGCCATCCACTGGAAGG | 371 | (ca)14(ca)14 |
| LUCA2.2 | GGCAGTGTTTGAGCTTACATGGG | CAGGCTCTGGAAACCAAGC | 172 | (ca)29 |
| LUCA4.1 | CTGCCTGCCTCACTACTCC | CAAACTGCCCAGCTTCTG | 182 | (ca)23 |
| LUCA4.2 | CCAGTGAGTACCAAGATATGGG | GAGAATGTGACCCGGGC | 236 | (aaac)6 |
| LUCA4.3 | ACATGGTGGCATGTGCC | AGACCTTTAGCGTGTCATTTAC | 239 | (aaaat)6 |
| LUCA4.4 | GATCATGGAGGCCTTGAATG | TGTTCATTGCCTGCATGG | 407 | (ca)13 |
| LUCA6.3 | AAAGTACAGGGCAGTGTCAG | AGAAGAAGAGTCCAGCAAGC | 480 | (aatg)6 |
| LUCA7.1 | GGAGATCACCTGGACACATC | TAAGTGCTAGGAGGCACTGC | 229 | (agcccc)7 |
| LUCA7.2 | TTGTGGGCACTTCTTGCTAG | ACAACATATGCAAGGCCCAG | 523 | (aatg)6 |
| LUCA8.1 | TAACCCTTGGCTTTGCTG | GGCTACTCTTCAACAAGATGC | 274 | (ca)30 |
| LUCA8.2 | GCTGAGAAATCTCAATTGTGGGTG | GGCTGCTGAGCAGTGTCAGAC | 125 | (ca)11 |
| LUCA8.3 | AATTCAGAACTGCGCCTTG | GCAGAGTGAGTAAAGGTTCACAG | 485 | (aat)25 |
| LUCA11.1 | CACACACATCAGGCCGC | CTGCTCACAACCCCCTACC | 252 | (ca)22 |
| LUCA11.2 | AAGACCAGCAAGGTGGC | TACTAGGGCTCCAGAGAGGG | 238 | (ca)13 |
| LUCA11.4 | CCTGTGCCCACTCCCTATCTC | CCCAGGCTGGGATGCCTTTGG | 177 | (ca)12 |
| LUCA12.1 | TTCTCTGAGGAATGACTCCTGG | AACAGCGCCTTATGATGGAG | 308 | (cca)8 |
| LUCA13.4 | CCGCCTTCCAAGTTTAAGTG | TCAACCTGGGAGGTGGG | 199 | (aat)7 |
| LUCA17.1 | GCTTGATCTTGGCTCACCTC | AGGATCACTTGGGCCTG | 374 | (aaat)8 |
| LUCA17.2 | GTGGGTGGATCATCAGGTC | GTGCCTACAATAGAGAGGGTC | 360 | (ttttg)6 |
| LUCA17.4 | GAACATGGAAGGAGGAGG | CTCGATCTATCTCTTGATCTCAG | 220 | (aaat)8 |
| LUCA19.1 | AGGCAGGCTATATTCAACCC | GAAAAAGCCCCGGAACC | 199 | (ta)15 |
| LUCA19.2 | AATTAGGACCTTCAGGAAACC | CCAGCAGATGGAGGTTGC | 204 | (aaag)6 |
| LUCA20.2 | TGTGGTCCCACCTAATCTGG | CACATGCGTGCACATGC | 304 | (aaag)11, (aagg)11 |
| LUCA22 | GAAGTGGTTCACCCTAAGATC | AAGGGAGCTGGCCTAGGAG | 235 | (ct)12 |
| 3938P1.2 | AAACCTAGTTGATGCCAGGC | AACTTCTGGGATACATGGGC | 413 | (aaac)6 |
| 3938P1.3 | TGCCAGAGCCCCAAAAC | AAATGGGGCCAGGTGTG | 416 | (ttttc)6 |
| 3938P1.4 | TAAACCACTAGGCCCGGC | AGGTGTGGTGTTGTGTGCC | 246 | (ttttg)6 |
| 3938P1.5 | TGTTCCCTTCCCTATAAACAGAT | GAAAGCAAGGAAGGCACATG | 167 | (ca)24 |
| 3938P1.6 | CATGGTGGCACACAACTG | CCTCACCTGCCTGATTTTTG | 310 | (aaat)6 |
| 3938P1.7 | AGCAGATAGACAGATAAAGAGAGG | TCAGCTTCTTTGTCCCTTTCAC | 211 | (10 mer)5 |
*
Oligo name represents the LUCA cosmid the primer maps to (see Appendix).
†
Sequences are listed in a 5′ to 3′ orientation.