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. 2008 Feb 20;3:4. doi: 10.1186/1750-2187-3-4

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Copyright © 2008 Ma et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Immunoblotting analysis of the levels of transduced proteins in osteoclasts with HA-antibody. Osteoclasts were transduced with the WASP peptides as shown below the figures. Osteoclast lysates (~200 μg) were immunoblotted with an antibody to HA to determine the levels of transduced proteins (Top panel). The immunoblot shown in the top panel was stripped and blotted with a GAPDH antibody for normalization (bottom panel). The results shown are representative of three independent experiments.