Table 4.
BLU, RASSF1A and MGMT genes hypermethylation in astrocytoma tumor samples.
| BLU | RASSF1A | MGMT | |||||||||||||
| Sample | MSP | MCA-MSP | MCA-Meth | MSP | MCA-MSP | MCA-Meth | Seq | MSP | MCA-MSP | MCA-Meth | Seq | ||||
| 7 | A III | U | U | 0% | U+M | U+M | <5% | U | U+M | U+M | <5% | ||||
| 8 | A III-IV | U | U | U | 0% | U+M | U+M | U+M | <5% | U+M | U | U+M | <5% | ||
| 12 | GBM | U | U | U | 0% | U+M | M | U+M | <5% | U | U | U | 14% | ||
| 13 | GBM | U+M | U+M | U | 22% | U+M | M | U+M | 20% | U | U | U | 0% | ||
| 26 | GBM | U+M | U | U | 0% | U+M | M | U+M | <5% | U | U | U | 0% | U | |
| 28 | GBM | U+M | U | U+M | <5% | U+M | M | U+M | 12% | M | U+M | M | 100% | M | |
| 30 | GBM | U+M | U+M | U+M | 65% | U+M | U+M | U+M | 72% | M | U+M | U+M | UM | ||
| 33 | A III | U+M | U+M | U+M | 57% | U+M | U+M | U+M | 46% | U+M | U+M | UM | UM | ||
| 45 | GBM | U | U | U | 0% | U+M | U+M | U+M | <5% | U+M | U | U | 0% | ||
| 46 | GBM | U | U | U | 0% | U+M | U+M | U+M | <5% | U+M | U | U+M | <5% | ||
| 47 | A III-IV | U | U | U | 0% | U+M | U+M | U+M | 22% | U+M | U | U+M | <5% | ||
| 49 | GBM | U | U | U | 0% | U+M | U+M | U+M | <5% | U | U | U | 0% | ||
| 51 | GBM | U | U | U | 16% | U+M | U+M | U+M | <5% | U+M | U | UM | |||
U, unmethylated peak or band; M, methylated peak or band; U+M, sample producing both methylated and unmethylated peaks or bands; MU, sample producing only one peak at an intermediate temperature; blank spaces: not determined analyses. The percentage of fully methylated DNA molecules present in the samples (semiquantitative methylation study) is shown for the MCA-Meth in the M and U+M cases. On the contrary, no percentage appears in the the UM cases, as the UM corresponds to partially methylated DNA molecules, that can not be semiquantitatively studied according to the curve obtained with different proportions of fully methylated DNA.