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EMSA with methylated CpG oligunucleotides. EMSA was carried out using nuclear extracts prepared from mtCC or Hepa 1 cells and oligonucleotides containing proximal or distal CpG site methylated (Me +) or unmethylated (Me -). Oligonucleotide sequences are shown at the bottom with methylated C underlined in boldface. Shifted bands only seen with methylated CpG oligonucleotide are shown by open arrowhead for proximal or asterisk for distal CpG region.
