Figure 4.

C/EBPβ bound to the aromatase promoter I.3/II region in situ in LSMCs. ChIP-PCR was performed using LSMCs treated with Bt₂cAMP to verify specific binding between C/EBP family proteins (C/EBPα, β, and δ) and the aromatase promoter I.3/II region in intact cells. Nonimmune rabbit IgG (IgG) was used as a negative control. A, Amplification of the −383/−257 bp region of aromatase promoter I.3/II, which contains a C/EBP binding site at −317/−304 bp. B, Amplification of the −275/−177 bp region of promoter I.3/II, which contains another C/EBP binding site at −245/−231 bp. Diagrams indicate the relative positions of each primer pair within the promoter I.3/II region. Input, Product before immunoprecipitation; TSS, transcription start site.