Skip to main content
. 2008 Jan 8;93(3):981–991. doi: 10.1210/jc.2007-2507

Table 1.

Oligonucleotide sequences used for PCR

Target gene Objective Primer pairs’ name: sequence (5′–3′) Amplicon size (bp)
Aromatase Real-time PCR Exon II F: CAC ATC CTC AAT ACC AGG TCC 143
Exon III R: CAG AGA TCC AGA CTC GCA TG
C/EBPβ Real-time PCR C/EBPβ 1588F: CGT GTG TAC ACG GGA CTG AC 86
C/EBPβ 1674R: CAA CAA GCC CGT AGG AAC AT
GAPDH Real-time PCR GAPDH F: GAA GGT GAA GGT CGG AGT C 224
GAPDH R: GAA GAT GGT GAT GGG ATT TC
Aromatase promoter I.3/II ChIP-PCR PII −383 F: TTG GTC AAAAAG GGG AGT TG 126
PII −257 R: AGC TCA TTC CAG AGG TGG AG
Aromatase promoter I.3/II ChIP-PCR PII −275 F: TCC ACC TCT GGA ATG AGC TT 98
PII −177 R: ATC ATC TTG CCC TTG AGT GG
Aromatase promoter Multiplex RT-PCR PII F: TCC CTT TGA TTT CCA CAG GAC TC 303
I.1 F: CTG TGC TCG GGA TCT TCC 312
I.2 F: GGC TTC CTG ACT TTC AAC AG 321
I.3 F: CCT TCT TTT GAC TTG TAA CCA 330
I.4 F: CCT TGT TTT GAC TTG TAA CCA 339
I.6 F: TAAATT GAT TGT CTT GCA CAG G 347
I.7 F: GAA GTA AGA CCG GAG AAA GGG 365
I.8 F: TCA TAT TGG GAG GAG CTT GG 373
Aromatase promoter Multiplex RT-PCR Exon III R: [5FAM] CTC CAT ACA CCC GGT TGT AG

F, Forward; R, reverse.