Table 1.
Oligonucleotide sequences used for PCR
| Target gene | Objective | Primer pairs’ name: sequence (5′–3′) | Amplicon size (bp) |
|---|---|---|---|
| Aromatase | Real-time PCR | Exon II F: CAC ATC CTC AAT ACC AGG TCC | 143 |
| Exon III R: CAG AGA TCC AGA CTC GCA TG | |||
| C/EBPβ | Real-time PCR | C/EBPβ 1588F: CGT GTG TAC ACG GGA CTG AC | 86 |
| C/EBPβ 1674R: CAA CAA GCC CGT AGG AAC AT | |||
| GAPDH | Real-time PCR | GAPDH F: GAA GGT GAA GGT CGG AGT C | 224 |
| GAPDH R: GAA GAT GGT GAT GGG ATT TC | |||
| Aromatase promoter I.3/II | ChIP-PCR | PII −383 F: TTG GTC AAAAAG GGG AGT TG | 126 |
| PII −257 R: AGC TCA TTC CAG AGG TGG AG | |||
| Aromatase promoter I.3/II | ChIP-PCR | PII −275 F: TCC ACC TCT GGA ATG AGC TT | 98 |
| PII −177 R: ATC ATC TTG CCC TTG AGT GG | |||
| Aromatase promoter | Multiplex RT-PCR | PII F: TCC CTT TGA TTT CCA CAG GAC TC | 303 |
| I.1 F: CTG TGC TCG GGA TCT TCC | 312 | ||
| I.2 F: GGC TTC CTG ACT TTC AAC AG | 321 | ||
| I.3 F: CCT TCT TTT GAC TTG TAA CCA | 330 | ||
| I.4 F: CCT TGT TTT GAC TTG TAA CCA | 339 | ||
| I.6 F: TAAATT GAT TGT CTT GCA CAG G | 347 | ||
| I.7 F: GAA GTA AGA CCG GAG AAA GGG | 365 | ||
| I.8 F: TCA TAT TGG GAG GAG CTT GG | 373 | ||
| Aromatase promoter | Multiplex RT-PCR | Exon III R: [5FAM] CTC CAT ACA CCC GGT TGT AG |
F, Forward; R, reverse.