Table 1.
Primer set and probe for the real-time PCR analysis of islet hormones and acinar transcripts
| cDNA | GenBank accession no. | Primer | Primer sequence (5′–3′) | Probe sequence (5′–3′) |
|---|---|---|---|---|
| INS | NM_000207 | F | GCAGCCTTTGTGAACCAACA | TGCGGCTCACACCTGGTGGAAG |
| R | TTCCCCGCACACTAGGTAGAGA | |||
| GCG | NM_002054 | F | CAAGGCAGCTGGCAACGT | CAGGCAGACCCACTCAGTGATCCTGAT |
| R | CTGGTGAATGTGCCCTGTGA | |||
| SST | NM_001048 | F | GATGCCCTGGAACCTGAAGA | CTGTCCCAGGCTGCTGAGCAGGA |
| R | CTGCAGCTCAAGCCTCATTTC | |||
| AMY2A | NM_000699 | F | ACTGGACATTTTCTTTAACTTTGCAA | TGGTCTTCCTGCTGGCACATACTGTGA |
| R | GCCATTAATTTTATCTCCAGAAATGAC | |||
| PRSS1 | NM_002769 | F | CATGTTCTGTGTGGGCTTCCT | AGGGAGGCAAGGATTCATGTCAGGGT |
| R | ACCACAGGGCCACCAGAAT | |||
| PRSS2 | NM_002770 | F | TCCTGCCAGGGTGATTCTG | TGGCCCTGTGGTCTCCAATGGAGA |
| R | ATAGCCCCAGGAGACAATTCC | |||
| ELA3A | NM_005747 | F | TGCCCCACAGAGGATGGT | AGCTTTGTTTCTGGCTTTGGCTGCAAC |
| R | CCGTGGGCTTCCAGATGA | |||
| CPA2 | NM_001869 | F | GCCAGCCCGTCAGATCCT | CCCACAGCCGAGGAGACCTGGC |
| R | ATGCTCCATGATTGCCTTCAA | |||
| CPB1 | NM_001871 | F | TCACTGCACGGCACCAAGT | CACATATGGCCCGGGAGCTACAACA |
| R | CCCCCAGCAGCAGGATAGA | |||
| PNLIP | NM_000936 | F | AGATTATAGTGGAGACAAATGTTGGAAA | CAGTTCAACTTCTGTAGTCCAGAAACCGTCAGG |
| R | GTGAGGGTGAGCAGAACTTCCT | |||
| CA2 | NM_000067 | F | ACAATTGTTGACTAAAATGCTGCTTT | TGGTTGAGTGCAAATCCATAGCACAAGATAAA |
| R | CTATTTTACCTGATTTGCCTTAACTAGCT | |||
| CK19 | NM_002276 | F | GAGTACCAGCGGCTCATGGA | CAAGTCGCGGCTGGAGCAGGA |
| R | GCTGCGGTAGGTGGCAAT | |||
| RPL32 | NM_000994 | F | CTGGCCATCAGAGTCACCAA | CCCAATGCCAGGCTGCGCA |
| R | TGAGCTGCCTACTCATTTTCTTCA |
Oligonucleotide sequences for forward (F) and reverse (R) primers are shown. RPL32, Ribosomal protein L32.