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. 2008 Mar 26;3(3):e1855. doi: 10.1371/journal.pone.0001855

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Tuikue Ndam et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) PCR products confirming splicing of intron 1 but not intron 2 on 3D7 and a placental isolate cDNA. Primers indicated on (A) by arrows were used to amplify full size and a short fragment of the gene on 3D7 genomic DNA (lane 2 and 5), and on cDNA of 3D7 (lane 3 and 6) and a placental isolate (lane 4 and 7). Lane 1 indicates a 100 bp DNA lader. (B) Diagram of exon/intron structures from predicted gene and mRNA/cDNA. The heavy bars are predicted exons. Empty and stripped bars are predicted intron 1 and intron 2 respectively according to 3D7 gDNA sequence. Figures below the gDNA structure indicate nucleotide positions. mRNA/cDNA structure is the experimentally observed structure.