Table 2. Confirmation by real-time quantitative rt-PCR of in vivo expression of mRNA for 13 different genes, compared with that in 3D7 in vitro reference.

Microarray fold change			Gene ID	rt-qPCR fold change
Group 1	Group 2	Group 3		Group 1	Group 2	Group 3
25,03	90,27	18,48	PFL0030c (+)	40,78	99,76	109,67
40,97	21,55	19,88	PFC0110w (+)	10,66	18,4	23,15
7,08	35,45	4,63	PFI1785w (+)	149,15	319,69	206,25
5,5	12,89	3,43	PF10_0351 (+)	5,56	6,63	5,03
5,61	12,74	3,1	PFA0700c (+)	103,7	171,37	112,99
5,61	3,74	3,54	PF14_0010 (+)	10,2	26,48	5,75
8,36	3,39	2,69	PF10_0344 (+)	2,76	3,81	1,91
3,31	2,93	4,59	PF14_0757 (+)	2,84	1,63	0,81
6,72	2,55		PFB0105c (+)	3,95	4,4	4
			PF13_0304 ( = )	0,94	1,2	0,72
	0,34	0,23	PFL0260c (−)	0,16	0,22	0,28
	0,32	0,17	PF10_0350 (−)	0,56	0,61	0,26
0,3	0,3	0,16	P FD1120c (−)	0,26	0,22	0,19

Gene's selection criteria were based on similar expression observed in at least 2 out 3 tests sample pools. Values are fold changes calculated as compared to the 3D7 level. Gene expression levels ranged from overexpressed in in vivo parasites to fairly expressed according to arrays measurements. (+) For over-expressed in placental parasites according to array data and (−) For under-expressed in placental parasites according to array data. One gene with no difference in expression ( = ) according to microarray was also included. Empty cells correspond to no difference observed.