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. 2008 Mar 7;4(3):e1000015. doi: 10.1371/journal.ppat.1000015

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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HeLa cells (top panel) were transfected with pNL4-3/MA-TC or 1GA or 29KE/31KE derivatives. Cells were labeled with FlAsH for 5 min at 37°C, washed, fixed in 3.7% formaldehyde, and examined microscopically. MDMs (bottom panel) were infected with VSV-G- pseudotyped virus stocks that transduced NL4-3/MA-TC or 1GA or 29KE/31KE derivatives (see Materials and Methods). Infected cells were labeled and fixed as described above. Scale bars = 30 µm. For a 3D z-series of the 29KE/31KE-TC mutant in MDM, see Video S1.