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. 2008 Mar 7;4(3):e1000015. doi: 10.1371/journal.ppat.1000015

Figure 3. Gag in MDMs colocalizes with tetraspanins at the cell surface and in apparently internal compartments.

Figure 3

MDMs infected with VSV-G-pseudotyped NL4-3/MA-TC (A and C) or NL4-3/29KE/31KE-TC (B) were labeled with FlAsH (green) 20 hours post-infection, fixed, and further labeled with antibody against CD63 (red) (A and B) or CD81 (red) (C). The merged images of Gag-TC and CD63/CD81 are shown on the right, with yellow indicating colocalization between Gag and CD63/CD81. R = Pearson coefficient of correlation. Scale bars: panel A, 40 µm; panel B, 30 µm; panel C; 30 µm top, 20 µm bottom.