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. 2008 Mar 7;4(3):e1000015. doi: 10.1371/journal.ppat.1000015

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) MDMs were labeled with FlAsH 20, 24, 48, 72, and 96 hrs after infection with VSV-G-pseudotyped NL4-3/MA-TC. At each time point, between 25 and 65 cells were categorized as having PM, intracellular (IC), or both PM+intracellular (PM+IC) Gag localization. (B) Live-cell analysis. MDMs infected with VSV-G-pseudotyped NL4-3/MA-TC virus were labeled with FlAsH 48 hrs postinfection, and immediately examined microscopically on the stage in a closed chamber (37°C/5% CO₂). Time (t) represents time in min after FlAsH labeling. Scale bar = 30 µm.