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. 2008 Mar 7;4(3):e1000015. doi: 10.1371/journal.ppat.1000015

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) MDMs infected with VSV-G-pseudotyped NL4-3/MA-TC virus were labeled with FlAsH 48 hrs postinfection. Jurkat T-cells were added to the FlAsH-labeled cultures and incubated on the microscope stage in a closed chamber (37°C /5% CO₂). Time t = 0 min is 40 min (gallery A) or 70 min (gallery B) after addition of Jurkat cells. M1 = infected MDM; M2 = non-infected MDM; T1 and T2 are Jurkat T-cells. Scale bars = 20 µm in A and 40 µm in B. For a movie of Gag movement to the synapse, derived from the experiment presented in Fig. 9A, see Video S7.